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human ace2 tmprss2 expressing a549 cells  (InvivoGen)


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    InvivoGen human ace2 tmprss2 expressing a549 cells
    Human Ace2 Tmprss2 Expressing A549 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human ace2 tmprss2 expressing a549 cells/product/InvivoGen
    Average 96 stars, based on 194 article reviews
    human ace2 tmprss2 expressing a549 cells - by Bioz Stars, 2026-02
    96/100 stars

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    a. To examine how many cells were infected by HCoV-OC43 at 24hpi, immunostaining using a monoclonal antibody targeting HCoV-OC43 was conducted (MAB9012). Here we show representative confocal images of Mock and HCoV-OC43 infected <t>A549-ACE2</t> cells stained with MAB9012 (red) and DAPI (blue). Scale bar is shown.
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    a. To examine how many cells were infected by HCoV-OC43 at 24hpi, immunostaining using a monoclonal antibody targeting HCoV-OC43 was conducted (MAB9012). Here we show representative confocal images of Mock and HCoV-OC43 infected <t>A549-ACE2</t> cells stained with MAB9012 (red) and DAPI (blue). Scale bar is shown.
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    Comparative fitness of EG.5.1 and XBB.1.9.1 (A) Beta-galactosidase staining on differentiated nasal organoid monolayers from a younger adult (28 years old) and an older adult (68 years old). Upper panel: light microscopy; Lower panel: Nuclei were counterstained with DAPI (blue). Scale bar, 100 μm (left), 50 μm (right). (B) Viral replication in VeroE6/TMPRSS2 cell line, A549/TMPRSS2/ACE2 cell line or nasal organoids derived from the younger adult or the older adult. The viral load was determined using RT-qPCR. Data represent mean, and error bar represents one standard deviation. The viral load at each time point was compared using Student’s t test. ∗, p < 0.05. (C) Competition assay between EG.5.1 and XBB.1.9.1. Human nasal organoid derived from the young adult was infected with EG.5.1 and XBB.1.9.1 at a EG.5.1:XBB.1.9.1 TCID 50 ratio of 1:1. Next generation sequencing was performed on the cell culture supernatants that were collected at 24, 48 and 72 hpi. The percentage of reads corresponding to the EG.5.1 was determined using the spike amino acid residues 52 and 456. The data at 0 h time point represents the sequencing result of the 1:1 mixture. Data represent mean, and error bar represents one standard deviation.

    Journal: iScience

    Article Title: Characterizing fitness and immune escape of SARS-CoV-2 EG.5 sublineage using elderly serum and nasal organoid

    doi: 10.1016/j.isci.2024.109706

    Figure Lengend Snippet: Comparative fitness of EG.5.1 and XBB.1.9.1 (A) Beta-galactosidase staining on differentiated nasal organoid monolayers from a younger adult (28 years old) and an older adult (68 years old). Upper panel: light microscopy; Lower panel: Nuclei were counterstained with DAPI (blue). Scale bar, 100 μm (left), 50 μm (right). (B) Viral replication in VeroE6/TMPRSS2 cell line, A549/TMPRSS2/ACE2 cell line or nasal organoids derived from the younger adult or the older adult. The viral load was determined using RT-qPCR. Data represent mean, and error bar represents one standard deviation. The viral load at each time point was compared using Student’s t test. ∗, p < 0.05. (C) Competition assay between EG.5.1 and XBB.1.9.1. Human nasal organoid derived from the young adult was infected with EG.5.1 and XBB.1.9.1 at a EG.5.1:XBB.1.9.1 TCID 50 ratio of 1:1. Next generation sequencing was performed on the cell culture supernatants that were collected at 24, 48 and 72 hpi. The percentage of reads corresponding to the EG.5.1 was determined using the spike amino acid residues 52 and 456. The data at 0 h time point represents the sequencing result of the 1:1 mixture. Data represent mean, and error bar represents one standard deviation.

    Article Snippet: TMPRSS2 and ACE2 over-expressing A549 cells (A549/TMPRSS2/ACE2) were obtained from InvivoGen (Catalog code: a549-hace2tpsa).

    Techniques: Staining, Light Microscopy, Derivative Assay, Quantitative RT-PCR, Standard Deviation, Competitive Binding Assay, Infection, Next-Generation Sequencing, Cell Culture, Sequencing

    Journal: iScience

    Article Title: Characterizing fitness and immune escape of SARS-CoV-2 EG.5 sublineage using elderly serum and nasal organoid

    doi: 10.1016/j.isci.2024.109706

    Figure Lengend Snippet:

    Article Snippet: TMPRSS2 and ACE2 over-expressing A549 cells (A549/TMPRSS2/ACE2) were obtained from InvivoGen (Catalog code: a549-hace2tpsa).

    Techniques: Virus, Recombinant, Modification, Saline, Plasmid Preparation, Staining, Sequencing, Software

    a. To examine how many cells were infected by HCoV-OC43 at 24hpi, immunostaining using a monoclonal antibody targeting HCoV-OC43 was conducted (MAB9012). Here we show representative confocal images of Mock and HCoV-OC43 infected A549-ACE2 cells stained with MAB9012 (red) and DAPI (blue). Scale bar is shown.

    Journal: Nature microbiology

    Article Title: SARS-CoV-2 restructures host chromatin architecture

    doi: 10.1038/s41564-023-01344-8

    Figure Lengend Snippet: a. To examine how many cells were infected by HCoV-OC43 at 24hpi, immunostaining using a monoclonal antibody targeting HCoV-OC43 was conducted (MAB9012). Here we show representative confocal images of Mock and HCoV-OC43 infected A549-ACE2 cells stained with MAB9012 (red) and DAPI (blue). Scale bar is shown.

    Article Snippet: Cell culture Human lung adenocarcinoma cells A549 expressing human ACE2 (A549-ACE2, #NR53821) was acquired from BEI Resources (Manassas, VA).

    Techniques: Infection, Immunostaining, Staining